One of our readers posted the following question to us and we decided to pass it along to everybody's favorite microbiology expert, Aunt Yersinia: For one year I am working in different research laboratories, after I got from school. I keep wondering why EVERYBODY is using pre-made Agar solutions for pouring plates, and EVERYBODY is heating it up the dirty way…
Dec 17, 2009· Dan gives a demo of how to pour agar plates. 1) Microwave the agar until melted 2) Cool down for 15-20min (until it's cool to touch) 3) Add antibiotics and swirl gently 4) Pour and let sit until .
Tip: Microbiologists typically use sterile, disposable polystyrene plates, but you can also use glass Petri dishes or even baby food jars. If you use glass dishes or jars, you may find it easier to sterilize the culture media directly in them. Prepare the media as in Step 3, but instead of sterilizing it, boil it until the agar is dissolved, then pour into individual containers.
Procedure. Prepare the Agar Plates: Loosen or remove the cap on the Nutrient agar bottle. Place the bottle in the microwave (if you do not have a microwave, place the bottle in a heat-safe bowl and pour boiling water around the bottle) and heat until the entire agar bottle is liquefied.
Sep 14, 2016· Open one plate at a time next to the flame and begin pouring. Measure your desired amount of agar with a pipete for the first plate to get a good idea of what that volume looks like in your particular plate. For the remainder of the plates, pour directly from the bottle.
remain at around 100°C. Leave it in the water bath until the agar is completely melted. While wearing heat-protective gloves, carefully remove the hot bottle and let it cool to between 75–55°C before pouring. c)Microwave: Loosen the cap on the agar bottle before microwaving. Heat in one minute intervals on low power until all of the agar is .
Apr 24, 2017· Agar is the gelatinous substance that sits inside the petri dishes used by scientists and students alike. Agar is the perfect substance for biological experiments as it holds up to bacteria and doesn't disintegrate easily. There are a number of ways to make an agar plate or agar-filled petri dish. You can purchase .